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h 4000 immunohistochemistry reagent other normal goat serum  (Vector Laboratories)


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    Vector Laboratories h 4000 immunohistochemistry reagent other normal goat serum
    H 4000 Immunohistochemistry Reagent Other Normal Goat Serum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 6362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+4000+immunohistochemistry+reagent+other+normal+goat+serum/Normal+Goat+Serum+Blocking+Solution/10__7554_slash_elife__96425__3-529-25-36
    Average 96 stars, based on 6362 article reviews
    h 4000 immunohistochemistry reagent other normal goat serum - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Immunohistochemistry:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Blocking Assay:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Protein Extraction:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Spatial Transcriptomics:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Infection:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.

    Staining:

    Article Title: Chemokine expression profile of an innate granuloma
    Article Snippet: DOI: https://doi.org/10.7554/eLife.96425 22 of 28 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 9 GraphPad (Boston, MA) Software, algorithm Fiji ImageJ (Burleson, TX) Other Collagenase Type IV Gibco Ref# 17104019 Tissue dissociation media Other 1× DMEM, +4.5 g/l D- Glucose, +L- Glutamine, +110 mg/l Sodium Pyruvate Gibco Ref# 11995- 065 Cell culture media Other 1× RPMI Medium 1640, +L- Glutamine Gibco Ref# 11875- 093 Cell culture media Other PenStrep +10,000 units/ml Penicillin, +10,000 μg/ml Streptomycin Gibco Ref# 15140- 122 Antibiotics Other HyClone Characterized Fetal Bovine Serum Cytiva (Marlborough, MA) Ref# SH30396.03 Cell culture media Other 1× DPBS, -Calcium Chloride, -Magnesium Chloride Gibco Ref# 14190- 144 Cell culture media Other 70 μm Cell Strainers Genesee Scientific (El Cajon, CA) Ref# 25- 376 Tissue dissociation reagent Other 40 μm Cell Strainers Genesee Scientific Ref# 25- 375 Tissue dissociation reagent Other Percoll GE Healthcare (Chicago, IL) Ref# 17- 0891- 01 Tissue dissociation reagent Other 1× RBC Lysis Buffer eBioscience Ref# 00- 4333- 57 Flow cytometry reagent Other Falcon Round- Bottom Polystyrene Test Tubes Thermo Scientific Ref# 14- 959- 1A Flow cytometry tubes Other Mouse BD Fc Block BD Biosciences Ref# 553142 Blocking reagent; used at 1 μg (FC), 2% (IF) Other Intracellular Fixation & Permeabilization Buffer eBioscience Ref# 88- 8824- 00 Flow cytometry reagent Other 10% Neutral Buffered Formalin VWR (Radnor, PA) Ref# 16004–128 Histology reagent Other 16% Paraformaldehyde VWR Ref# 15710S Immunofluorescence reagent Other Sucrose Sigma- Aldrich Ref# S1888 Immunofluorescence reagent Other Tissue- Tek O.C.T. .. Compound Sakura (Torrance, CA) Ref# 4583 Immunofluorescence reagent Other Epredia Xylene Fisher Chemical Ref# 99- 905- 01 Immunohistochemistry reagent Other ImmEdge Pen Vector Laboratories Ref# H- 4000 Immunohistochemistry reagent Other Normal Goat Serum Blocking Solution, 2.5% Vector Laboratories Ref# S- 1012 Immunohistochemistry reagent Other SignalStain Antibody Diluent Cell Signaling Ref# 8112 Immunohistochemistry reagent Other Permount Fisher Chemical Ref# SP15- 100 Immunohistochemistry reagent Other T- PER Tissue Protein Extraction Reagent Thermo Scientific Ref# 78510 Tissue dissociation reagent Other Sulfuric Acid Ricca Chemical (Arlington, TX) Ref# 8310- 32 ELISA Stop Buffer Other Fluoroshield with DAPI Sigma- Aldrich Ref# F6057 Immunofluorescence reagent Continued Analysis of spatial transcriptomics dataset Tissues from infected mice were harvested at the indicated timepoints, which were chosen based on key events observed via H&E staining (Harvest et al., 2023). .. Spatial data were generated in Harvest et al., 2023 using the 10X Genomics Visium Platform.



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